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Dev Cell. 2015 Mar 23;32(6):772-4. doi: 10.1016/j.devcel.2015.03.004.

Reassessment of Piwi binding to the genome and Piwi impact on RNA polymerase II distribution.

Author information

1
Yale Stem Cell Center, Yale University, New Haven, CT 06520, USA; Department of Cell Biology, Yale University, New Haven, CT 06520, USA. Electronic address: haifan.lin@yale.edu.
2
Department of Biostatistics, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC 27599, USA.
3
Department of Genetics, Stanford University, Stanford, CA 94305, USA; Department of Computer Science, Stanford University, Stanford, CA 94305, USA.
4
Division of Bioinformatics, Department of Preventive Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, USA.
5
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.
6
Yale Stem Cell Center, Yale University, New Haven, CT 06520, USA; Department of Cell Biology, Yale University, New Haven, CT 06520, USA.
7
Department of Genetics, Stanford University, Stanford, CA 94305, USA.

Abstract

Drosophila Piwi was reported by Huang et al. (2013) to be guided by piRNAs to piRNA-complementary sites in the genome, which then recruits heterochromatin protein 1a and histone methyltransferase Su(Var)3-9 to the sites. Among additional findings, Huang et al. (2013) also reported Piwi binding sites in the genome and the reduction of RNA polymerase II in euchromatin but its increase in pericentric regions in piwi mutants. Marinov et al. (2015) disputed the validity of the Huang et al. bioinformatic pipeline that led to the last two claims. Here we report our independent reanalysis of the data using current bioinformatic methods. Our reanalysis agrees with Marinov et al. (2015) that Piwi's genomic targets still remain to be identified but confirms the Huang et al. claim that Piwi influences RNA polymerase II distribution in the genome. This Matters Arising Response addresses the Marinov et al. (2015) Matters Arising, published concurrently in this issue of Developmental Cell.

PMID:
25805139
PMCID:
PMC4472434
DOI:
10.1016/j.devcel.2015.03.004
[Indexed for MEDLINE]
Free PMC Article
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