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Nat Commun. 2015 Jan 20;6:5998. doi: 10.1038/ncomms6998.

Comprehensive bioimaging with fluorinated nanoparticles using breathable liquids.

Author information

1
Scripps Center for Metabolomics and Mass Spectrometry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
2
Pfizer Worldwide Research and Development, 10724 Science Center Dr, San Diego, CA 92121, USA.
3
Departments of Chemical Physiology and Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
4
Department of Molecular and Cellular Neuroscience, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
5
Waters Corporation, 100 Cummings Center, Beverly, Massachusetts 01915, USA.
6
Department of Microbiology and Immunology and Center for Biofilm Engineering, Montana State University, 109 Lewis Hall, Montana State University, Bozeman, Montana 59717, USA.
7
Department of Cell and Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
8
The Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, Nebraska 68198-5880, USA.
9
The Core Microscopy Facility, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
10
1] Department of Chemistry, Washington University in St. Louis, One Brookings Drive, St. Louis, Missouri 63130, USA [2] Departments Genetics and Medicine, Washington University School of Medicine, 660 South Euclid Ave., St Louis, Missouri 63110, USA.
11
1] Scripps Center for Metabolomics and Mass Spectrometry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA [2] Departments of Chemistry, Molecular and Computational Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

Abstract

Fluorocarbons are lipophobic and non-polar molecules that exhibit remarkable biocompatibility, with applications in liquid ventilation and synthetic blood. The unique properties of these compounds have also enabled mass spectrometry imaging of tissues where the fluorocarbons act as a Teflon-like coating for nanostructured surfaces to assist in desorption/ionization. Here we report fluorinated gold nanoparticles (f-AuNPs) designed to facilitate nanostructure imaging mass spectrometry. Irradiation of f-AuNPs results in the release of the fluorocarbon ligands providing a driving force for analyte desorption. The f-AuNPs allow for the mass spectrometry analysis of both lipophilic and polar (central carbon) metabolites. An important property of AuNPs is that they also act as contrast agents for X-ray microtomography and electron microscopy, a feature we have exploited by infusing f-AuNPs into tissue via fluorocarbon liquids to facilitate multimodal (molecular and anatomical) imaging.

PMID:
25601659
PMCID:
PMC4454288
DOI:
10.1038/ncomms6998
[Indexed for MEDLINE]
Free PMC Article

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