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Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):17206-11. doi: 10.1073/pnas.1420193111. Epub 2014 Nov 17.

Reducing the genetic code induces massive rearrangement of the proteome.

Author information

  • 1Departments of Biochemistry and Chemistry, The University of Western Ontario, London, ON N6A 5C1, Canada;
  • 2Departments of Molecular Biophysics and Biochemistry.
  • 3Department of Microbiology, Technical University of Braunschweig, Braunschweig 38106, Germany; and.
  • 4Division of Microbial Physiology and Molecular Biology, University of Greifswald, Greifswald 17487, Germany.
  • 5Cellular and Molecular Physiology, and Systems Biology Institute, Yale University, New Haven, CT 06520;
  • 6Departments of Molecular Biophysics and Biochemistry, Chemistry, and dieter.soll@yale.edu ilka.heinemann@uwo.ca.
  • 7Departments of Biochemistry and dieter.soll@yale.edu ilka.heinemann@uwo.ca.

Abstract

Expanding the genetic code is an important aim of synthetic biology, but some organisms developed naturally expanded genetic codes long ago over the course of evolution. Less than 1% of all sequenced genomes encode an operon that reassigns the stop codon UAG to pyrrolysine (Pyl), a genetic code variant that results from the biosynthesis of Pyl-tRNA(Pyl). To understand the selective advantage of genetically encoding more than 20 amino acids, we constructed a markerless tRNA(Pyl) deletion strain of Methanosarcina acetivorans (ΔpylT) that cannot decode UAG as Pyl or grow on trimethylamine. Phenotypic defects in the ΔpylT strain were evident in minimal medium containing methanol. Proteomic analyses of wild type (WT) M. acetivorans and ΔpylT cells identified 841 proteins from >7,000 significant peptides detected by MS/MS. Protein production from UAG-containing mRNAs was verified for 19 proteins. Translation of UAG codons was verified by MS/MS for eight proteins, including identification of a Pyl residue in PylB, which catalyzes the first step of Pyl biosynthesis. Deletion of tRNA(Pyl) globally altered the proteome, leading to >300 differentially abundant proteins. Reduction of the genetic code from 21 to 20 amino acids led to significant down-regulation in translation initiation factors, amino acid metabolism, and methanogenesis from methanol, which was offset by a compensatory (100-fold) up-regulation in dimethyl sulfide metabolic enzymes. The data show how a natural proteome adapts to genetic code reduction and indicate that the selective value of an expanded genetic code is related to carbon source range and metabolic efficiency.

KEYWORDS:

evolution; genetic code expansion; methanogenesis; pyrrolysine; tRNAPyl

PMID:
25404328
PMCID:
PMC4260545
DOI:
10.1073/pnas.1420193111
[PubMed - indexed for MEDLINE]
Free PMC Article
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