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J Infect Dis. 2015 Apr 1;211(7):1174-84. doi: 10.1093/infdis/jiu573. Epub 2014 Nov 2.

Prolonged proinflammatory cytokine production in monocytes modulated by interleukin 10 after influenza vaccination in older adults.

Author information

1
Section of Infectious Diseases, Department of Internal Medicine.
2
Department of Center for Vaccine Development, Saint Louis University, Missouri.
3
Department of Pathology.
4
Department of Laboratory Medicine.
5
Department of Neurology.
6
Department of Neurology Department of Immunobiology.
7
Department of Immunobiology.
8
Department of Pathology Department of Interdepartmental Program in Computational Biology and Bioinformatics, Yale University, New Haven, Connecticut.
9
Department of Internal Medicine, Yale School of Medicine.

Abstract

We evaluated in vivo innate immune responses in monocyte populations from 67 young (aged 21-30 years) and older (aged ≥65 years) adults before and after influenza vaccination. CD14(+)CD16(+) inflammatory monocytes were induced after vaccination in both young and older adults. In classical CD14(+)CD16(-) and inflammatory monocytes, production of tumor necrosis factor α and interleukin 6, as measured by intracellular staining, was strongly induced after vaccination. Cytokine production was strongly associated with influenza vaccine antibody response; the highest levels were found as late as day 28 after vaccination in young subjects and were substantially diminished in older subjects. Notably, levels of the anti-inflammatory cytokine interleukin 10 (IL-10) were markedly elevated in monocytes from older subjects before and after vaccination. In purified monocytes, we found age-associated elevation in phosphorylated signal transducer and activator of transcription-3, and decreased serine 359 phosphorylation of the negative IL-10 regulator dual-specificity phosphatase 1. These findings for the first time implicate dysregulated IL-10 production in impaired vaccine responses in older adults.

KEYWORDS:

aging; cytokine; influenza vaccine; innate immunity; monocyte

PMID:
25367297
PMCID:
PMC4366602
DOI:
10.1093/infdis/jiu573
[Indexed for MEDLINE]
Free PMC Article
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