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Sci Rep. 2014 Oct 29;4:6816. doi: 10.1038/srep06816.

Reversal of anchorage-independent multicellular spheroid into a monolayer mimics a metastatic model.

Author information

1
Russell H. Morgan Department of Radiology and Radiological Sciences.
2
Microscope Facility, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Abstract

Lack of an in vitro model of metastasis has been a major impediment in understanding the molecular regulation of metastatic processes, and identification of specific therapeutic targets. We have established an in vitro model which displayed the signatures of metastatic phenotype such as migration, invasiveness, chemoresistance and expression of cancer stem-cell markers. This in vitro model was developed by the induction of reversal of multicellular spheroids that were generated by anchorage-independent growth. In vivo data further validated the metastatic phenotype of the in vitro model. Besides delineating the molecular events of metastasis, this model could also improve the screening efficiency of antimetastatic agents.

PMID:
25351825
PMCID:
PMC4212233
DOI:
10.1038/srep06816
[Indexed for MEDLINE]
Free PMC Article
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