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J Clin Microbiol. 2014 Dec;52(12):4385-7. doi: 10.1128/JCM.02635-14. Epub 2014 Oct 8.

A highly sensitive europium nanoparticle-based immunoassay for detection of influenza A/B virus antigen in clinical specimens.

Author information

1
Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA.
2
Laboratory of Molecular Virology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA saivikram.vemula@fda.hhs.gov indira.hewlett@fda.hhs.gov.
3
Center for Devices and Radiological Health, Food and Drug Administration, Silver Spring, Maryland, USA.
4
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut, USA.

Abstract

We report the development of a novel europium nanoparticle-based immunoassay (ENIA) for rapid detection of influenza A and influenza B viruses. The ENIA demonstrated sensitivities of 90.7% (147/162) for influenza A viruses and 81.80% (9/11) for influenza B viruses compared to those for an in-house reverse transcription (RT)-PCR assay in testing of influenza-positive clinical samples.

PMID:
25297327
PMCID:
PMC4313323
DOI:
10.1128/JCM.02635-14
[Indexed for MEDLINE]
Free PMC Article
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