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Nat Nanotechnol. 2014 Aug;9(8):639-47. doi: 10.1038/nnano.2014.154. Epub 2014 Aug 3.

A carbon nanotube-polymer composite for T-cell therapy.

Author information

1
Department of Chemical Engineering, Yale University, PO Box 208284, New Haven, Connecticut 06511, USA.
2
Department of Biomedical Engineering, Yale University, PO Box 208284, New Haven, Connecticut 06511, USA.
3
1] Department of Immunobiology and Internal Medicine, Yale University, PO Box 208284, New Haven, Connecticut 06520, USA [2].
4
Department of Immunobiology and Microbiology, Blegdamsvej 3b DK2200, Copenhagen N Denmark.
5
Department of Immunobiology and Internal Medicine, Yale University, PO Box 208284, New Haven, Connecticut 06520, USA.
6
1] Department of Chemical Engineering, Yale University, PO Box 208284, New Haven, Connecticut 06511, USA [2] Department of Biomedical Engineering, Yale University, PO Box 208284, New Haven, Connecticut 06511, USA [3] Department of Immunobiology and Internal Medicine, Yale University, PO Box 208284, New Haven, Connecticut 06520, USA.

Erratum in

  • Nat Nanotechnol. 2014 Sep;9(9):723. Harold, Kevin C [corrected to Herold, Kevan C].

Abstract

Clinical translation of cell therapies requires strategies that can manufacture cells efficiently and economically. One promising way to reproducibly expand T cells for cancer therapy is by attaching the stimuli for T cells onto artificial substrates with high surface area. Here, we show that a carbon nanotube-polymer composite can act as an artificial antigen-presenting cell to efficiently expand the number of T cells isolated from mice. We attach antigens onto bundled carbon nanotubes and combined this complex with polymer nanoparticles containing magnetite and the T-cell growth factor interleukin-2 (IL-2). The number of T cells obtained was comparable to clinical standards using a thousand-fold less soluble IL-2. T cells obtained from this expansion were able to delay tumour growth in a murine model for melanoma. Our results show that this composite is a useful platform for generating large numbers of cytotoxic T cells for cancer immunotherapy.

PMID:
25086604
DOI:
10.1038/nnano.2014.154
[Indexed for MEDLINE]

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