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Methods Mol Biol. 2014;1176:115-26. doi: 10.1007/978-1-4939-0992-6_10.

Arginine-grafted biodegradable polymer: a versatile transfection reagent for both DNA and siRNA.

Author information

1
Department of Internal Medicine, Section of Infectious Diseases, Yale University School of Medicine, 25 York Street, New Haven, CT, 06520, USA.

Abstract

Effective delivery of DNA or siRNA into primary cells demands an efficient delivery system. However, the significant differences in physical and molecular characteristics of the two molecules generally necessitate distinct delivery systems or considerable differences in carrier formulation protocols for effective transfection. Arginine-grafted bioreducible poly (disulfide amine) (ABP) is a redox-sensitive, bioreducible, positively charged polymer which complexes with siRNA and DNA via charge interactions to form nanoplexes. ABP effectively mediates cytoplasmic delivery of both DNA and siRNA into multiple cell types, including primary cells like myoblast, human umbilical vein endothelial cells (HUVECs), and primary rat aorta vascular smooth muscle cells (SMCs) eliciting functional activity. In this chapter, we provide the detailed protocols for the synthesis of ABP as well as transfection of both siRNA and DNA using ABP.

PMID:
25030923
DOI:
10.1007/978-1-4939-0992-6_10
[Indexed for MEDLINE]

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