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Methods Mol Biol. 2014;1176:89-106. doi: 10.1007/978-1-4939-0992-6_8.

Targeted genome modification via triple helix formation.

Author information

1
Department of Biomedical Engineering, Yale University, New Haven, CT, USA.

Abstract

Triplex-forming oligonucleotides (TFOs) are capable of coordinating genome modification in a targeted, site-specific manner, causing mutagenesis or even coordinating homologous recombination events. Here, we describe the use of TFOs such as peptide nucleic acids for targeted genome modification. We discuss this method and its applications and describe protocols for TFO design, delivery, and evaluation of activity in vitro and in vivo.

PMID:
25030921
PMCID:
PMC5111905
DOI:
10.1007/978-1-4939-0992-6_8
[Indexed for MEDLINE]
Free PMC Article

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