Format

Send to

Choose Destination
J Cell Sci. 2014 Aug 15;127(Pt 16):3625-40. doi: 10.1242/jcs.154997. Epub 2014 Jun 24.

The KASH protein Kms2 coordinates mitotic remodeling of the spindle pole body.

Author information

1
Department of Cell Biology, Yale School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA.
2
Department of Cell Biology, Yale School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA megan.king@yale.edu.

Abstract

Defects in the biogenesis of the spindle pole body (SPB), the yeast centrosome equivalent, can lead to monopolar spindles and mitotic catastrophe. The KASH domain protein Kms2 and the SUN domain protein Sad1 colocalize within the nuclear envelope at the site of SPB attachment during interphase and at the spindle poles during mitosis in Schizosaccharomyces pombe. We show that Kms2 interacts with the essential SPB components Cut12 and Pcp1 and the Polo kinase Plo1. Depletion of Kms2 delays mitotic entry and leads to defects in the insertion of the SPB into the nuclear envelope, disrupting stable bipolar spindle formation. These effects are mediated in part by a delay in the recruitment of Plo1 to the SPB at mitotic entry. Plo1 activity supports mitotic SPB remodeling by driving a burst of incorporation of Cut12 and Pcp1. Thus, a fission yeast SUN-KASH complex plays an important role in supporting the remodeling of the SPB at mitotic entry.

KEYWORDS:

LINC complex; Nuclear envelope; Spindle pole body

PMID:
24963130
PMCID:
PMC4132395
DOI:
10.1242/jcs.154997
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center