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J Clin Microbiol. 2014 Aug;52(8):3057-9. doi: 10.1128/JCM.00738-14. Epub 2014 May 21.

Comparison of Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR in predominantly adult hospitalized patients.

Author information

1
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut, USA Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, USA Clinical Virology Laboratory, Yale-New Haven Hospital, New Haven, Connecticut, USA marie.landry@yale.edu.
2
Clinical Virology Laboratory, Yale-New Haven Hospital, New Haven, Connecticut, USA.

Abstract

To compare Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR methods, 445 nasopharyngeal samples were tested. Of these, 199 were positive (46 for respiratory syncytial virus [RSV], 120 for influenza A, and 33 for influenza B) and 246 were negative. The direct fluorescent-antibody assay (DFA) detected 132 (66.3%) positive samples, Simplexa direct detected 162 (81.4%), Simplexa using extracts detected 177 (88.9%), and lab-developed TaqMan PCR reference methods detected 199 (100%). The specificities were 99.6%, 100%, 100%, and 100%, respectively. The two Simplexa methods were more sensitive than the DFA (P = 0.0001) but less sensitive than the TaqMan reverse transcriptase PCR (P = 0.0001).

PMID:
24850350
PMCID:
PMC4136128
DOI:
10.1128/JCM.00738-14
[Indexed for MEDLINE]
Free PMC Article
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