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Am J Physiol Renal Physiol. 2014 Jul 1;307(1):F64-74. doi: 10.1152/ajprenal.00547.2013. Epub 2014 May 7.

Role of medullary progenitor cells in epithelial cell migration and proliferation.

Author information

1
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut; and.
2
Department of Urology, Xiangya Hospital, Central South University, Changsha, China.
3
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut; and gilbert.moeckel@yale.edu.

Abstract

This study is aimed at characterizing medullary interstitial progenitor cells and to examine their capacity to induce tubular epithelial cell migration and proliferation. We have isolated a progenitor cell side population from a primary medullary interstitial cell line. We show that the medullary progenitor cells (MPCs) express CD24, CD44, CXCR7, CXCR4, nestin, and PAX7. MPCs are CD34 negative, which indicates that they are not bone marrow-derived stem cells. MPCs survive >50 passages, and when grown in epithelial differentiation medium develop phenotypic characteristics of epithelial cells. Inner medulla collecting duct (IMCD3) cells treated with conditioned medium from MPCs show significantly accelerated cell proliferation and migration. Conditioned medium from PGE2-treated MPCs induce tubule formation in IMCD3 cells grown in 3D Matrigel. Moreover, most of the MPCs express the pericyte marker PDGFR-b. Our study shows that the medullary interstitium harbors a side population of progenitor cells that can differentiate to epithelial cells and can stimulate tubular epithelial cell migration and proliferation. The findings of this study suggest that medullary pericyte/progenitor cells may play a critical role in collecting duct cell injury repair.

KEYWORDS:

CD24; CXCR4; pericyte; progenitor cell

PMID:
24808539
PMCID:
PMC4080159
DOI:
10.1152/ajprenal.00547.2013
[Indexed for MEDLINE]
Free PMC Article
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