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Mol Genet Genomic Med. 2014 Mar;2(2):160-5. doi: 10.1002/mgg3.55. Epub 2014 Feb 5.

High frequency of exon 15 deletion in the FANCA gene in Tunisian patients affected with Fanconi anemia disease: implication for diagnosis.

Author information

1
Department of Histology and Cytogenetics, Institut Pasteur de Tunis Tunis, Tunisia ; Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, Tunis El Manar University Tunis, Tunisia.
2
Laboratory of Biomedical Genomics and Oncogenetics, Institut Pasteur de Tunis, Tunis El Manar University Tunis, Tunisia.
3
Department of Tumour Biology, Institut Curie Paris, France.
4
Haematology Department, Hedi Chaker Hospital, University of Sfax Sfax, Tunisia.
5
Department of Haematology and Transplantation, National Bone Marrow Transplantation Centre Tunis, Tunisia.
6
Department of Peadiatric Immuno-Haematology, National Bone Marrow Transplantation Tunis, Tunisia.
7
Haematology Department, Aziza Othmana Hospital Tunis, Tunisia.
8
Department of Tumour Biology, Institut Curie Paris, France ; Institut Curie, INSERM U830 Paris, France ; Sorbonne Paris Cité, Université Paris Descartes Paris, France.
9
Hôpital Saint-Louis Paris, France.
10
Department of Pediatrics, CHU Hedi Chaker Sfax, Tunisia.
11
Laboratory of Transmission, Immunology and Infection Control, Institut Pasteur de Tunis Tunis, Tunisia.
12
Department of Histology and Cytogenetics, Institut Pasteur de Tunis Tunis, Tunisia.

Abstract

Tunisian population is characterized by its heterogeneous ethnic background and high rate of consanguinity. In consequence, there is an increase in the frequency of recessive genetic disorders including Fanconi anemia (FA). The aim of this study was to confirm the existence of a founder haplotype among FA Tunisian patients and to identify the associated mutation in order to develop a simple tool for FA diagnosis. Seventy-four unrelated families with a total of 95 FA patients were investigated. All available family members were genotyped with four microsatellite markers flanking FANCA gene. Haplotype analysis and homozygosity mapping assigned 83 patients belonging to 62 families to the FA-A group. A common haplotype was shared by 42 patients from 26 families at a homozygous state while five patients from five families were heterozygous. Among them, 85% were from southern Tunisia suggesting a founder effect. Using multiplex ligation-dependent probe amplification (MLPA) technique, we have also demonstrated that this haplotype is associated with a total deletion of exon 15 in FANCA gene. Identification of a founder mutation allowed genetic counseling in relatives of these families, better bone marrow graft donor selection and prenatal diagnosis. This mutation should be investigated in priority for patients originating from North Africa and Middle East.

KEYWORDS:

Exon 15 deletion; Fanconi anemia; MLPA; founder haplotype; founder mutation

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