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Methods. 2014 Jun 15;68(1):207-17. doi: 10.1016/j.ymeth.2014.01.005. Epub 2014 Jan 17.

Methods for studying oogenesis.

Author information

1
Department of Genetics, Yale University School of Medicine, United States.
2
Department of Genetics, Yale University School of Medicine, United States; Department of Cell Biology, Yale University School of Medicine, United States; Department of Molecular, Cellular & Developmental Biology, Yale University, United States. Electronic address: lynn.cooley@yale.edu.

Abstract

Drosophila oogenesis is an excellent system for the study of developmental cell biology. Active areas of research include stem cell maintenance, gamete development, pattern formation, cytoskeletal regulation, intercellular communication, intercellular transport, cell polarity, cell migration, cell death, morphogenesis, cell cycle control, and many more. The large size and relatively simple organization of egg chambers make them ideally suited for microscopy of both living and fixed whole mount tissue. A wide range of tools is available for oogenesis research. Newly available shRNA transgenic lines provide an alternative to classic loss-of-function F2 screens and clonal screens. Gene expression can be specifically controlled in either germline or somatic cells using the Gal4/UAS system. Protein trap lines provide fluorescent tags of proteins expressed at endogenous levels for live imaging and screening backgrounds. This review provides information on many available reagents and key methods for research in oogenesis.

KEYWORDS:

Drosophila; Genetics; Live-cell imaging; Oogenesis; RNAi

PMID:
24440745
PMCID:
PMC4048766
DOI:
10.1016/j.ymeth.2014.01.005
[Indexed for MEDLINE]
Free PMC Article
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