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Biochem Biophys Res Commun. 2014 Jan 3;443(1):74-9. doi: 10.1016/j.bbrc.2013.11.056. Epub 2013 Nov 19.

Saponin monomer 13 of dwarf lilyturf tuber (DT-13) protects serum withdrawal-induced apoptosis through PI3K/Akt in HUVEC.

Author information

1
State Key Laboratory of Natural Medicines, Department of Complex Prescription of TCM, China Pharmaceutical University, 639 Longmian Road, Nanjing 211198, PR China; Department of Internal Medicine, Section of Cardiovascular Medicine, Yale University School of Medicine, New Haven, CT 06519, USA; Vascular Biology and Therapeutics Program, Yale University School of Medicine, New Haven, CT 06519, USA.
2
Vascular Biology and Therapeutics Program, Yale University School of Medicine, New Haven, CT 06519, USA.
3
State Key Laboratory of Natural Medicines, Department of Complex Prescription of TCM, China Pharmaceutical University, 639 Longmian Road, Nanjing 211198, PR China. Electronic address: boyangyucpu@163.com.
4
Department of Internal Medicine, Section of Cardiovascular Medicine, Yale University School of Medicine, New Haven, CT 06519, USA; Vascular Biology and Therapeutics Program, Yale University School of Medicine, New Haven, CT 06519, USA. Electronic address: jun.yu@yale.edu.

Abstract

Dwarf lilyturf tuber is widely used in clinics to prevent cardiovascular diseases. DT-13, the saponin monomer 13 of dwarf lilyturf tuber, shows protective activities in anti-thrombosis, anti-inflammation, and cardioprotective. However, little is known about the cellular function of DT-13 in cardiovascular system. Vascular endothelial cells (EC) are important to maintain the integrity of the vasculature throughout entire body. Dysregulation of EC may lead to pathophysiological processes of numerous cardiovascular diseases. We thus tested the function of DT-13 in EC. In the present study, we are the first to report that DT-13 has anti-apoptosis activity on human umbilical vein endothelial cells (HUVEC), potentially through down regulation of cleaved caspase-3 and cleaved PARP expression. DT-13 also increased mitochondrial membrane potential. To explore the potential mechanism, we investigated the effect of DT-13 on Akt and MAPK pathways and found that DT-13 was involved in Akt signaling confirmed by using PI3K/Akt inhibitor LY294002. Thus, DT-13 could improve survival of EC and therefore be a potential clinical use in the treatment of cardiovascular diseases.

KEYWORDS:

Akt; Apoptosis; DT-13; EC

PMID:
24269237
PMCID:
PMC4736752
DOI:
10.1016/j.bbrc.2013.11.056
[Indexed for MEDLINE]
Free PMC Article

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