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PLoS One. 2013 Oct 8;8(10):e76807. doi: 10.1371/journal.pone.0076807. eCollection 2013.

Breeding based remobilization of Tol2 transposon in Xenopus tropicalis.

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1
Program in Vertebrate Developmental Biology, Department of Pediatrics and Genetics, Yale University School of Medicine, New Haven, Connecticut, United States of America.

Abstract

Xenopus is a powerful model for studying a diverse array of biological processes. However, despite multiple methods for transgenesis, relatively few transgenic reporter lines are available and commonly used. Previous work has demonstrated that transposon based strategies are effective for generating transgenic lines in both invertebrate and vertebrate systems. Here we show that the Tol2 transposon can be remobilized in the genome of X. tropicalis and passed through the germline via a simple breeding strategy of crossing transposase expressing and transposon lines. This remobilization system provides another tool to exploit transgenesis and opens new opportunities for gene trap and enhancer trap strategies.

PMID:
24116167
PMCID:
PMC3792888
DOI:
10.1371/journal.pone.0076807
[Indexed for MEDLINE]
Free PMC Article
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