Format

Send to

Choose Destination
Radiat Res. 2013 Jun;179(6):674-683. doi: 10.1667/RR3198.1. Epub 2013 May 3.

Functional proteomics analysis to study ATM dependent signaling in response to ionizing radiation.

Author information

1
Department of Oncology, Georgetown University Medical Center, Washington, DC.
2
Department of Radiation Medicine, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC.
#
Contributed equally

Abstract

Ataxia telangiectasia (AT) is a human genetic disease characterized by radiation sensitivity, impaired neuronal development and predisposition to cancer. Using a genetically defined model cell system consisting of cells expressing a kinase dead or a kinase proficient ATM gene product, we previously reported systemic alterations in major metabolic pathways that translate at the gene expression, protein and small molecule metabolite levels. Here, we report ionizing radiation induced stress response signaling arising from perturbations in the ATM gene, by employing a functional proteomics approach. Functional pathway analysis shows robust translational and post-translational responses under ATM proficient conditions, which include enrichment of proteins in the Ephrin receptor and axonal guidance signaling pathways. These molecular networks offer a hypothesis generating function for further investigations of cellular stress responses.

PMID:
23642045
PMCID:
PMC4301981
DOI:
10.1667/RR3198.1
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioOne Icon for PubMed Central
Loading ...
Support Center