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Bioessays. 2013 May;35(5):452-61. doi: 10.1002/bies.201200131. Epub 2013 Mar 27.

Microtubule dynamic instability: a new model with coupled GTP hydrolysis and multistep catastrophe.

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1
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.

Erratum in

  • Bioessays. 2013 Jun;35(6):579.

Abstract

A key question in understanding microtubule dynamics is how GTP hydrolysis leads to catastrophe, the switch from slow growth to rapid shrinkage. We first provide a review of the experimental and modeling literature, and then present a new model of microtubule dynamics. We demonstrate that vectorial, random, and coupled hydrolysis mechanisms are not consistent with the dependence of catastrophe on tubulin concentration and show that, although single-protofilament models can explain many features of dynamics, they do not describe catastrophe as a multistep process. Finally, we present a new combined (coupled plus random hydrolysis) multiple-protofilament model that is a simple, analytically solvable generalization of a single-protofilament model. This model accounts for the observed lifetimes of growing microtubules, the delay to catastrophe following dilution and describes catastrophe as a multistep process.

PMID:
23532586
PMCID:
PMC3677417
DOI:
10.1002/bies.201200131
[Indexed for MEDLINE]
Free PMC Article
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