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Methods Mol Biol. 2013;965:373-82. doi: 10.1007/978-1-62703-239-1_25.

Genome-wide RNAi screening to identify regulators of oncogene-induced cellular senescence.

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Department of Pathology, Yale University School of Medicine, New Haven, CT, USA.


RNA interference (RNAi) is a powerful research tool that can be used to turn off-or silence-the expression of a specific gene. In recent years, RNAi screening on a genome-wide scale has provided the opportunity to identify factors and pathways involved in complex biological processes in a systematic and unbiased manner. Here we describe a genome-wide RNAi screening strategy to identify genes that are required for a particular type of cellular senescence called oncogene-induced senescence. The approach we describe is a general screening strategy that can be applied to the study of other forms of cellular senescence, including replicative senescence.

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