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ACS Chem Biol. 2013 Jan 18;8(1):122-6. doi: 10.1021/cb300490j. Epub 2012 Oct 24.

Transcriptional silencing by single-stranded RNAs targeting a noncoding RNA that overlaps a gene promoter.

Abstract

RNAi using single-strand RNA would provide new options for therapeutic development and for investigating critical questions of mechanism. Using chemically modified single-strands, we test the hypothesis that single-stranded RNAs can engage the RNAi pathway and silence gene transcription. We find that a chemically modified single-stranded silencing RNA (ss-siRNA) designed to be complementary to a long noncoding RNA (lncRNA) requires argonaute protein, functions through the RNAi pathway, and inhibits gene transcription. These data expand the use of single-stranded RNA to cell nuclei.

PMID:
23082936
PMCID:
PMC3909965
DOI:
10.1021/cb300490j
[Indexed for MEDLINE]
Free PMC Article

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