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Chembiochem. 2012 Nov 5;13(16):2327-30. doi: 10.1002/cbic.201200531. Epub 2012 Sep 24.

Sulfonyl fluoride analogues as activity-based probes for serine proteases.

Author information

1
Department of Chemistry, Boston College, Chestnut Hill, MA 02467, USA.

Abstract

Enriched with fluoride: To expand on the available tools to interrogate proteases, we explored sulfonyl fluorides as activity-based probes. An alkyne-tagged sulfonyl fluoride covalently modifies members of the S1 family of serine proteases. By applying click chemistry, avidin enrichment and mass spectrometry, we can enrich and identify active endogenous serine proteases from a complex proteome.

PMID:
23008217
DOI:
10.1002/cbic.201200531
[Indexed for MEDLINE]

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