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J Membr Biol. 2012 Nov;245(11):667-74. doi: 10.1007/s00232-012-9425-7. Epub 2012 Jun 23.

Expression, purification and functional reconstitution of slack sodium-activated potassium channels.

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  • 1Department of Cellular and Molecular Physiology, Yale University, New Haven, CT 06520-8026, USA.

Abstract

The slack (slo2.2) gene codes for a potassium-channel α-subunit of the 6TM voltage-gated channel family. Expression of slack results in Na(+)-activated potassium channel activity in various cell types. We describe the purification and reconstitution of Slack protein and show that the Slack α-subunit alone is sufficient for potassium channel activity activated by sodium ions as assayed in planar bilayer membranes and in membrane vesicles.

PMID:
22729647
PMCID:
PMC3903048
DOI:
10.1007/s00232-012-9425-7
[PubMed - indexed for MEDLINE]
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