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Rev Peru Med Exp Salud Publica. 2012 Mar;29(1):69-75.

[Experimental efficacy of IgY antibodies produced in eggs against the venom of the Peruvian snake Bothrops atrox].

[Article in Spanish]

Author information

1
Laboratorio de Biología Molecular, Universidad Nacional Mayor de San Marcos, Lima, Perú. sfexryu@gmail.com

Abstract

OBJECTIVES:

To develop an immunization protocol in order to produce avian IgY immunoglobulins against Bothrops atrox Peruvian snake venom and to evaluate its neutralizing capacity.

MATERIALS AND METHODS:

Six Hy Line Brown hens were immunized each two weeks using 500μg/doses of B. atrox venom in a period of two months. Each week, eggs were collected for IgY isolation from yolk using two consecutive steps with caprilic acid and ammonium sulfate. Detection of IgY anti-B. atrox were performed by double immunodiffusion, whereas title and cross-reactivity were analyzed using ELISA and Western Blot technics, respectively. Furthermore, letal dose (DL(50)) and Medium Effective Dose (DE(50)) were obtained by Probit analysis.

RESULTS:

As a result of this protocol, chicken IgY's were obtained in a concentration of 8,5 ± 1,35 mg/yolk mL. DE50 from avian antivenom was 575 μL/venom mg. Cross-reactivity studies showed Bothrops atrox venom share more commom epitopes with Bothrops brazili (47%) than others Bothrops venoms showing Lachesis muta (19%) and Crotalus durissus (12%) venoms a low crossing reactivity, instead.

CONCLUSIONS:

Using this procedure, we could purify chicken IgY with a neutralizant capacity of B. atrox venom which is comparable to the antivenom of equine origin and demonstrate its capacity as a immunoanalitical tool to evaluate the cross reactivity with others peruvian snakes.

PMID:
22510909
DOI:
10.1590/s1726-46342012000100010
[Indexed for MEDLINE]
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