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DNA Repair (Amst). 2012 Apr 1;11(4):381-90. doi: 10.1016/j.dnarep.2012.01.004. Epub 2012 Feb 15.

The human gastric cancer-associated DNA polymerase β variant D160N is a mutator that induces cellular transformation.

Author information

1
Department of Therapeutic Radiology, Yale School of Medicine, New Haven, CT 06520, United States.

Abstract

Approximately 30% of human tumors sequenced to date harbor mutations in the POLB gene that are not present in matched normal tissue. Many mutations give rise to enzymes that contain non-synonymous single amino acid substitutions, several of which have been found to have aberrant activity or fidelity and transform cells when expressed. The DNA Polymerase β (Pol β) variant Asp160Asn (D160N) was first identified in a gastric tumor. Expression of D160N in cells induces cellular transformation as measured by hyperproliferation, focus formation, anchorage-independent growth and invasion. Here, we show that D160N is an active mutator polymerase that induces complex mutations. Our data support the interpretation that complex mutagenesis is the underlying mechanism of the observed cellular phenotypes, all of which are linked to tumorigenesis or tumor progression.

PMID:
22341651
PMCID:
PMC3624760
DOI:
10.1016/j.dnarep.2012.01.004
[Indexed for MEDLINE]
Free PMC Article

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