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Chembiochem. 2012 Mar 5;13(4):538-41. doi: 10.1002/cbic.201100793. Epub 2012 Jan 23.

Identification of hydrophobic tags for the degradation of stabilized proteins.

Author information

1
Department of Molecular Cellular and Developmental Biology, Yale University, 219 Prospect Street, New Haven, CT 06511, USA.

Abstract

New HyTs are a knockout: we previously reported that labeling HaloTag proteins with low molecular weight hydrophobic tags (HyTs) leads to targeted degradation of HaloTag fusion proteins. In this report, we employed a chemical approach to extend this hydrophobic tagging methodology to highly stabilized proteins by synthesizing and evaluating a library of HyTs, which led to the identification of HyT36.

PMID:
22271667
PMCID:
PMC3338156
DOI:
10.1002/cbic.201100793
[Indexed for MEDLINE]
Free PMC Article

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