Send to

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2012;833:153-76. doi: 10.1007/978-1-61779-477-3_11.

Monitoring dynamic binding of chromatin proteins in vivo by fluorescence recovery after photobleaching.

Author information

  • 1Groupe Imagerie et Mod√©lisation, Institut Pasteur, CNRS, URA 2582, Paris, France.


Fluorescence recovery after photobleaching (FRAP) has now become widely used to investigate nuclear protein binding to chromatin in live cells. FRAP can be applied qualitatively to assess if chromatin binding interactions are altered by various biological perturbations. It can also be applied semi-quantitatively to allow numerical comparisons between FRAP curves, and even fully quantitatively to yield estimates of in vivo diffusion constants and nuclear protein binding rates to chromatin. Here we describe how FRAP data should be collected and processed for these qualitative, semi-quantitative, and quantitative analyses.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Support Center