Format

Send to

Choose Destination
See comment in PubMed Commons below
Cell. 2011 Aug 19;146(4):582-92. doi: 10.1016/j.cell.2011.06.053.

Rapid microtubule self-assembly kinetics.

Author information

1
Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.

Erratum in

  • Cell. 2014 Sep 25;159(1):215.

Abstract

Microtubule assembly is vital for many fundamental cellular processes. Current models for microtubule assembly kinetics assume that the subunit dissociation rate from a microtubule tip is independent of free subunit concentration. Total-Internal-Reflection-Fluorescence (TIRF) microscopy experiments and data from a laser tweezers assay that measures in vitro microtubule assembly with nanometer resolution, provides evidence that the subunit dissociation rate from a microtubule tip increases as the free subunit concentration increases. These data are consistent with a two-dimensional model for microtubule assembly, and are explained by a shift in microtubule tip structure from a relatively blunt shape at low free concentrations to relatively tapered at high free concentrations. We find that because both the association and the dissociation rates increase at higher free subunit concentrations, the kinetics of microtubule assembly are an order-of-magnitude higher than currently estimated in the literature.

PMID:
21854983
PMCID:
PMC3171214
DOI:
10.1016/j.cell.2011.06.053
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center