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Methods Mol Biol. 2011;778:11-8. doi: 10.1007/978-1-61779-261-8_2.

Functional surface attachment in a sandwich geometry of GFP-labeled motor proteins.

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1
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.

Abstract

Molecular motors perform work in cells by moving in an ATP-dependent manner along filamentous tracks. In vitro, the mechanical action of such motor proteins can be investigated by attaching the molecules to surfaces in the so-called gliding or bead assays. Surface attachment protocols have to be used that do not interfere with the function of the molecule. Here, we describe a sandwich protocol that preserves functionality. The protocol can be used for a large variety of proteins, in particular kinesin motor proteins that are GFP-tagged.

PMID:
21809197
DOI:
10.1007/978-1-61779-261-8_2
[Indexed for MEDLINE]
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