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Appl Environ Microbiol. 2010 Nov;76(22):7635-40. doi: 10.1128/AEM.01188-10. Epub 2010 Sep 17.

Stable, site-specific fluorescent tagging constructs optimized for burkholderia species.

Author information

1
Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.

Abstract

Several vectors that facilitate stable fluorescent labeling of Burkholderia pseudomallei and Burkholderia thailandensis were constructed. These vectors combined the effectiveness of the mini-Tn7 site-specific transposition system with fluorescent proteins optimized for Burkholderia spp., enabling bacterial tracking during cellular infection.

PMID:
20851961
PMCID:
PMC2976199
DOI:
10.1128/AEM.01188-10
[Indexed for MEDLINE]
Free PMC Article

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