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Science. 2010 Jun 18;328(5985):1563-6. doi: 10.1126/science.1187197.

Down-regulation of a host microRNA by a Herpesvirus saimiri noncoding RNA.

Author information

1
Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.

Erratum in

  • Science. 2010 Sep 17;329(5998):1467. Steitz, Joan [corrected to Steitz, Joan A].

Abstract

T cells transformed by Herpesvirus saimiri express seven viral U-rich noncoding RNAs of unknown function called HSURs. We noted that conserved sequences in HSURs 1 and 2 constitute potential binding sites for three host-cell microRNAs (miRNAs). Coimmunoprecipitation experiments confirmed that HSURs 1 and 2 interact with the predicted miRNAs in virally transformed T cells. The abundance of one of these miRNAs, miR-27, is dramatically lowered in transformed cells, with consequent effects on the expression of miR-27 target genes. Transient knockdown and ectopic expression of HSUR 1 demonstrate that it directs degradation of mature miR-27 in a sequence-specific and binding-dependent manner. This viral strategy illustrates use of a ncRNA to manipulate host-cell gene expression via the miRNA pathway.

PMID:
20558719
PMCID:
PMC3075239
DOI:
10.1126/science.1187197
[Indexed for MEDLINE]
Free PMC Article

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