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J Clin Endocrinol Metab. 2010 Aug;95(8):3684-92. doi: 10.1210/jc.2010-0295. Epub 2010 May 19.

Comparative validation of the growth hormone-releasing hormone and arginine test for the diagnosis of adult growth hormone deficiency using a growth hormone assay conforming to recent international recommendations.

Author information

1
Unité Mixte de Recherche S693, Faculté de Médecine Paris-Sud, Université Paris-Sud 11, Institut National de la Santé et de la Recherce Médicale Unité 693, Service d'Endocrinologie et des Maladies de la Reproduction, Hôpital de Bicêtre, Assistance Publique-Hôpitaux de Paris, 78 Rue du Général Leclerc, Le Kremlin-Bicêtre 94275, France. philippe.chanson@bct.aphp.fr

Abstract

CONTEXT:

The GHRH plus arginine (GHRH+Arg) test is a promising alternative to the insulin tolerance test (ITT) for diagnosis of adult GH deficiency (AGHD).

OBJECTIVES:

The objectives of the study were to validate the GHRH+Arg test for diagnosis of AGHD, using the ITT as comparator and a GH assay calibrated according to recent international recommendations, and to study the repeatability and tolerance of both tests.

DESIGN:

This was a multicenter, randomized, open-label, phase III study.

SETTING:

The study was conducted at 10 French university hospitals.

SUBJECTS:

Sixty-nine subjects (38 and 15 with high and low probability of GH deficiency, respectively, and 16 healthy controls) were randomized: 35 to the GHRH+Arg-GHRH+Arg-ITT test sequence and 34 to the ITT-ITT-GHRH+Arg test sequence.

INTERVENTIONS:

Each subject underwent three tests of GH secretion separated by 24 h or more.

MAIN OUTCOME MEASURES:

The primary variable used for response assessments was serum peak GH response. Test results were compared with the final AGHD diagnosis.

RESULTS:

Peak GH responses in the two tests were strongly correlated. A cutoff value of 7.89 microg/liter for GHRH+Arg corresponding to 3 microg/liter for ITT was calculated. The cutoff value leading to 95% specificity with the GHRH+Arg test was measured at about 3.67 microg/liter (sensitivity 79.0%). Intermethod agreement and repeatability were high. Both tests were well tolerated. A preference for the GHRH+Arg test was expressed by 74% of subjects.

CONCLUSIONS:

The GHRH+Arg test demonstrated good accuracy and repeatability, was at least as sensitive as the ITT, and was associated with better subject acceptability. The GHRH+Arg test represents a good alternative to the ITT for the diagnosis of AGHD.

PMID:
20484474
DOI:
10.1210/jc.2010-0295
[Indexed for MEDLINE]

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