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J Clin Microbiol. 2010 Mar;48(3):957-9. doi: 10.1128/JCM.01678-09. Epub 2009 Dec 30.

Cytospin-enhanced immunofluorescence and impact of sample quality on detection of novel swine origin (H1N1) influenza virus.

Author information

1
Clinical Virology Laboratory, Yale New Haven Hospital, and Department of Laboratory Medicine,Yale University School of Medicine, New Haven, Connecticut 06520-8035, USA. marie.landry@yale.edu

Abstract

Nasopharyngeal swabs (n = 601) from 278 adult and 323 pediatric patients were tested within 24 h of receipt by cytospin-enhanced direct immunofluorescence antibody testing (DFA) and real-time reverse transcriptase PCR (RT-PCR) using the CDC assay. Cytospin-enhanced DFA detected 230 (84.6%) of 272 swine influenza A PCR-positive results overall but 25 (92.6%) of 27 positive results in patients less than 5 years old and 208 (96.7%) of 215 positive samples with cycle threshold values of <26.

PMID:
20042632
PMCID:
PMC2832425
DOI:
10.1128/JCM.01678-09
[Indexed for MEDLINE]
Free PMC Article

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