Format

Send to

Choose Destination
J Virol. 2010 Feb;84(3):1625-30. doi: 10.1128/JVI.01747-09. Epub 2009 Nov 11.

Viral mutagenesis as a means for generating novel proteins.

Author information

1
Department of Neurosurgery, Yale University School of Medicine, 333 Cedar St., New Haven, CT 06520, USA.

Abstract

We demonstrate that a mutation-prone virus engineered to express a foreign gene is an expedient means for generating novel mutant nonviral proteins in mammalian cells. Using vesicular stomatitis virus to express a gene coding for a fluorescent DsRed protein, a number of green mutant variants including a new variant not previously described were rapidly isolated from infected cells, sequenced, and cloned. Similar methods may be useful in the development of physiologically sensitive fluorescent reporter proteins and directed evolution or mutagenesis of proteins in general.

PMID:
19906913
PMCID:
PMC2812339
DOI:
10.1128/JVI.01747-09
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center