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Curr Opin Struct Biol. 2009 Oct;19(5):498-506. doi: 10.1016/j.sbi.2009.05.005. Epub 2009 Jul 3.

Mass spectrometry in the analysis of N-linked and O-linked glycans.

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  • 1Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK.

Abstract

Mass spectrometry (MS) continues to play a vital role in defining the structures of N-glycans and O-glycans in glycoproteins via glycomic and glycoproteomic methodologies. The former seeks to define the total N-glycan and/or O-glycan repertoire in a biological sample whilst the latter is concerned with the analysis of glycopeptides. Recent technical developments have included improvements in tandem mass spectrometry (MS/MS and MS(n)) sequencing methodologies, more sensitive methods for analysing sulfated and polysialylated glycans and better procedures for defining the sites of O-glycosylation. New tools have been introduced to assist data handling and publicly accessible databases are being populated with glycomics data. Progress is exemplified by recent research in the fields of glycoimmunology, reproductive glycobiology, stem cells, bacterial glycosylation and non-mucin O-glycosylation.

PMID:
19577919
PMCID:
PMC2965404
DOI:
10.1016/j.sbi.2009.05.005
[PubMed - indexed for MEDLINE]
Free PMC Article
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