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Dev Dyn. 2009 Jun;238(6):1398-46. doi: 10.1002/dvdy.21965.

Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes.

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1
Department of Pediatrics and Genetics, Yale University School of Medicine, New Haven, Connecticut, USA.

Abstract

Pilot forward genetic screens in Xenopus tropicalis have isolated over 60 recessive mutations. Here we present a simple method for mapping mutations to chromosomes using gynogenesis and centromeric markers. When coupled with available genomic resources, gross mapping facilitates evaluation of candidate genes as well as higher resolution linkage studies. Using gynogenesis, we have mapped the genetic locations of the 10 X. tropicalis centromeres, and performed fluorescence in situ hybridization to validate these locations cytologically. We demonstrate the use of this very small set of centromeric markers to map mutations efficiently to specific chromosomes. Developmental Dynamics 238:1398-1406, 2009. (c) 2009 Wiley-Liss, Inc.

PMID:
19441086
PMCID:
PMC2962985
DOI:
10.1002/dvdy.21965
[Indexed for MEDLINE]
Free PMC Article
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