Format

Send to

Choose Destination
Structure. 2008 Nov 12;16(11):1702-13. doi: 10.1016/j.str.2008.08.014.

Insights into the nature of DNA binding of AbrB-like transcription factors.

Author information

1
Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC 27695, USA.

Abstract

Understanding the DNA recognition and binding by the AbrB-like family of transcriptional regulators is of significant interest since these proteins enable bacteria to elicit the appropriate response to diverse environmental stimuli. Although these "transition-state regulator" proteins have been well characterized at the genetic level, the general and specific mechanisms of DNA binding remain elusive. We present RDC-refined NMR solution structures and dynamic properties of the DNA-binding domains of three Bacillus subtilis transition-state regulators: AbrB, Abh, and SpoVT. We combined previously investigated DNase I footprinting, DNA methylation, gel-shift assays, and mutagenic and NMR studies to generate a structural model of the complex between AbrBN(55) and its cognate promoter, abrB8. These investigations have enabled us to generate a model for the specific nature of the transition-state regulator-DNA interaction, a structure that has remained elusive thus far.

PMID:
19000822
PMCID:
PMC2606041
DOI:
10.1016/j.str.2008.08.014
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center