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Curr Protoc Cell Biol. 2003 Aug;Chapter 20:Unit 20.7. doi: 10.1002/0471143030.cb2007s19.

Optimization of transfection.

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Yale University School of Medicine, New Haven, Connecticut, USA.


When embarking upon any transfection procedure, a critical first step is to optimize conditions. Every mammalian cell type has a characteristic set of requirements for optimal introduction of foreign DNA; there is a tremendous degree of variability in the transfection conditions that work, even among cell types that are very similar to one another. Often, an experimenter must screen a wide variety of cell types for a desired regulatory trait, such as an appropriate response to a particular effector molecule. It is thus helpful to have a straightforward, systematic approach to optimizing transfection efficiency. This unit provides guidelines to optimizing transfection experiments.

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