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Curr Protoc Cell Biol. 2002 Feb;Chapter 16:Unit 16.6. doi: 10.1002/0471143030.cb1606s13.

Production of antibodies that recognize specific tyrosine-phosphorylated peptides.

Author information

1
Yale University School of Medicine, New Haven, Connecticut, USA.

Abstract

Phosphorylation of tyrosine residues of various proteins plays an important role in regulation of the functional activities of proteins within the cell. Antibodies to specific tyrosine-phosphorylated peptides are a valuable tool for analysis of the localization and function of individual phosphoproteins. This unit describes the methods used to produce and screen monoclonal and polyclonal antibodies for specificity for only the phosphorylated state of a peptide.

PMID:
18228399
DOI:
10.1002/0471143030.cb1606s13
[Indexed for MEDLINE]

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