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J Clin Microbiol. 2008 Mar;46(3):1098-100. doi: 10.1128/JCM.01926-07. Epub 2008 Jan 9.

Prospective study of human metapneumovirus detection in clinical samples by use of light diagnostics direct immunofluorescence reagent and real-time PCR.

Author information

1
Department of Laboratory Medicine, Yale New Haven Hospital, and Yale University School of Medicine, New Haven, CT 06520-8035, USA. marie.landry@yale.edu

Abstract

A Light Diagnostics human metapneumovirus (HMPV) monoclonal antibody reagent was evaluated using cytospin-enhanced direct immunofluorescence (DFA), and the results were compared to those for TaqMan reverse transcription-PCR (RT-PCR). Of the 202 samples tested, 48 were positive by RT-PCR, and 41 (85.4%) of these were positive by DFA (P = 0.0771). The commercial availability of an HMPV DFA reagent will be of significant benefit to clinical laboratories.

PMID:
18184854
PMCID:
PMC2268359
DOI:
10.1128/JCM.01926-07
[Indexed for MEDLINE]
Free PMC Article

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