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Cell. 2006 Dec 1;127(5):941-54.

Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis.

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1
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Abstract

New structures of RNA polymerase II (pol II) transcribing complexes reveal a likely key to transcription. The trigger loop swings beneath a correct nucleoside triphosphate (NTP) in the nucleotide addition site, closing off the active center and forming an extensive network of interactions with the NTP base, sugar, phosphates, and additional pol II residues. A histidine side chain in the trigger loop, precisely positioned by these interactions, may literally "trigger" phosphodiester bond formation. Recognition and catalysis are thus coupled, ensuring the fidelity of transcription.

PMID:
17129781
PMCID:
PMC1876690
DOI:
10.1016/j.cell.2006.11.023
[Indexed for MEDLINE]
Free PMC Article
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