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Proc Natl Acad Sci U S A. 2006 Jul 25;103(30):11417-22. Epub 2006 Jul 14.

Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls.

Author information

1
Carnegie Institution, Stanford, CA 94305, USA.

Abstract

To facilitate analysis of plant cell wall polysaccharide structure and composition, we cloned 74 genes encoding polysaccharide-degrading enzymes from Aspergillus nidulans, Aspergillus fumigatus, and Neurospora crassa and expressed the genes as secreted proteins with C-terminal Myc and 6x His tags. Most of the recombinant enzymes were active in enzyme assays, and optima for pH and temperature were established. A subset of the enzymes was used to fragment polysaccharides from the irregular xylem 9 (irx9) mutant of Arabidopsis. The analysis revealed a decrease in the abundance of xylan in the mutant, indicating that the IRX9 gene, which encodes a putative family 43 glycosyltransferase, is required for xylan synthesis.

PMID:
16844780
PMCID:
PMC1544100
DOI:
10.1073/pnas.0604632103
[Indexed for MEDLINE]
Free PMC Article

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