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Cell Calcium. 2006 Jan;39(1):65-73. Epub 2005 Nov 9.

Calcium release from ryanodine receptors in the nucleoplasmic reticulum.

Author information

1
Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USA.

Erratum in

  • Cell Calcium. 2006 Jun;39(6):551.

Abstract

Ca(2+) signals control DNA synthesis and repair, gene transcription, and other cell functions that occur within the nucleus. The nuclear envelope can store Ca(2+) and release it into the nucleus via either the inositol 1,4,5-trisphosphate receptor (InsP3R) or the ryanodine receptor (RyR). Furthermore, many cell types have a reticular network within their nuclei and InsP3Rs on this nucleoplasmic reticulum permit local subnuclear control of Ca(2+) signals and Ca(2+)-dependent intranuclear events. However, it is unknown whether RyR similarly is expressed on the nucleoplasmic reticulum and can control subnuclear Ca(2+) signals. Here we report that the type 1 RyR is expressed on intranuclear extensions of the sarcoplasmic reticulum of C2C12 cells, a skeletal muscle derived cell line. In addition, two-photon photorelease of caged Ca(2+) in the region of the nucleoplasmic reticulum evoked Ca(2+)-induced Ca(2+) release (CICR) within the nucleus, which could be suppressed by the RyR inhibitor dantrolene. These results show that intranuclear extensions of the nuclear envelope have functional RyR and provide a possible mechanism whereby cells expressing RyR can regulate Ca(2+) signals in discrete regions within the nucleus.

PMID:
16289270
DOI:
10.1016/j.ceca.2005.09.010
[Indexed for MEDLINE]

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