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Proc Natl Acad Sci U S A. 2004 Dec 28;101(52):17976-81. Epub 2004 Dec 17.

Splicing of U12-type introns deposits an exon junction complex competent to induce nonsense-mediated mRNA decay.

Author information

1
Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.

Abstract

Metazoan cells have two pathways for intron removal involving the U2- and U12-type spliceosomes, which contain mostly nonoverlapping sets of small nuclear ribonucleoproteins. We show that in vitro splicing of a U12-type intron assembles an exon junction complex (EJC) that is comparably positioned and contains many of the same components as that deposited by the U2-type spliceosome. The presence of a U12-type intron downstream of a premature termination codon within an open reading frame (ORF) induces nonsense-mediated decay of the mRNA in vivo. These findings suggest a common pathway for EJC assembly by the two spliceosomes and highlight the evolutionary age of the EJC and its downstream functions in gene expression.

PMID:
15608055
PMCID:
PMC539812
DOI:
10.1073/pnas.0408435102
[Indexed for MEDLINE]
Free PMC Article

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