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Acta Crystallogr D Biol Crystallogr. 2003 Nov;59(Pt 11):2031-8. Epub 2003 Oct 23.

Measurement errors and their consequences in protein crystallography.

Author information

1
Department of Biochemistry, UT Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9038, USA.

Abstract

This article analyzes the relative impact of various types of measurement uncertainties on different stages of structure determination. The treatment of errors is an important part of the experimental process and becomes critical when data quality is barely sufficient to solve and/or answer detailed questions about the structure. The sources and types of experimental errors are described and methods of minimizing their impact are discussed. Practical calculations of sigma estimates in DENZO and SCALEPACK are presented.

PMID:
14573959
[Indexed for MEDLINE]

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