Format

Send to

Choose Destination
J Clin Microbiol. 2003 Jul;41(7):3407-9.

Suboptimal detection of influenza virus in adults by the Directigen Flu A+B enzyme immunoassay and correlation of results with the number of antigen-positive cells detected by cytospin immunofluorescence.

Author information

1
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520, and Clinical Virology Laboratory, Yale New Haven Hospital, New Haven, Connecticut 06504, USA. marie.landry@yale.edu

Abstract

To provide 24-h influenza diagnosis for adults presenting to the emergency department, the Directigen Flu A+B enzyme immunoassay (EIA) was performed in the chemistry laboratory during the night shift. Nasopharyngeal swabs were retested by cytospin-enhanced direct immunofluorescence (DFA; SimulFluor respiratory screen) when the virology laboratory opened. The influenza EIA detected 16 influenza A virus infections, whereas cytospin-enhanced DFA detected 31 influenza A virus infections as well as 3 respiratory syncytial virus, 2 adenovirus, and 1 parainfluenza virus infections. A positive EIA result usually correlated with 50 or more influenza virus cells positive by DFA.

PMID:
12843105
PMCID:
PMC165325
DOI:
10.1128/jcm.41.7.3407-3409.2003
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center