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FEBS Lett. 2000 Sep 8;481(1):77-80.

Heterologous expression of a mammalian epithelial sodium channel in yeast.

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  • 1Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510, USA.

Abstract

The alpha and beta subunits of the amiloride-sensitive rat epithelial sodium channel (alpha beta ENaC) were expressed in the yeast Saccharomyces cerevisiae. We used a combination of yeast strains, including a mutant in the secretory pathway (sec6), and Western blotting techniques, to show that alpha beta ENaC was synthesized and targeted through the secretory system to the plasma membrane. Yeasts expressing alpha beta ENaC were more sensitive to salt than the parent strain. In addition, amiloride, a specific blocker of ENaC, was found to suppress salt sensitivity in the yeast strain expressing alpha beta ENaC.

PMID:
10984619
[PubMed - indexed for MEDLINE]
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