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Biochem Biophys Res Commun. 1999 Oct 14;264(1):112-8.

Molecular cloning of the gene for p85 that regulates the initiation of cytokinesis in Tetrahymena.

Author information

1
Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan.

Erratum in

  • Biochem Biophys Res Commun 2000 Jan 7;267(1):478.

Abstract

Tetrahymena p85 is localized to the presumptive division plane before division furrow formation; its molecular weight in SDS-polyacrylamide gel electrophoresis differs in wild-type and temperature-sensitive cell-division-arrest mutant cdaA1 cells. At the restrictive temperature, p85 localization and division furrow formation are not observed in cdaA1 cells. In this study, we purified p85 and cloned a wild-type p85 cDNA. The deduced amino acid sequence of p85 was composed mainly of two kinds of repeat sequences. One of these contained regions homologous to a calmodulin-binding site and a part of actin, and the other contained a region homologous to a part of a cdc2 kinase homologue. Moreover, we cloned a cDNA encoding the cdaA1 p85. There was no difference in the predicted amino acid sequences of wild-type and cdaA1 p85, suggesting that the difference in molecular weight between p85 in wild-type and mutant cells is caused by a disorder of posttranslational-modification mechanisms of p85 in the cdaA1 cell.

PMID:
10527850
DOI:
10.1006/bbrc.1999.1354
[Indexed for MEDLINE]

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