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Int J Syst Evol Microbiol. 2018 Oct;68(10):3285-3291. doi: 10.1099/ijsem.0.002980. Epub 2018 Aug 29.

Listeria goaensis sp. nov.

Author information

1
1​Institute for Medical Microbiology, Justus-Liebig University, and German Center for Infection Research (DZIF), Partner Site Gießen-Marburg-Langen, Campus Gießen, Germany.
2
2​Department of Pathology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India.
3
3​Department of Biotechnology, Goa University, Taleigao Plateau, Goa 403 206, India.
4
4​Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar 243122, India.
5
5​Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, and German Center for Infection Research (DZIF), Partner Site Hannover-Braunschweig, Braunschweig, Germany.
6
6​ICAR-National Research Centre on Meat, Chengicherla, Hyderabad 500092, India.

Abstract

Two Listeria-like isolates obtained from mangrove swamps in Goa, India were characterized using polyphasic combinations of phenotypic, chemotaxonomic and whole-genome sequence (WGS)-based approaches. The isolates presented as short, non-spore-forming, Gram-positive rods, that were non-motile, oxidase-negative, catalase-positive and exhibited α-haemolysis on 5 % sheep- and horse-blood agar plates. The 16S rRNA gene sequences exhibited 93.7-99.7 % nucleotide identity to other Listeria species and had less than 92 % nucleotide identity to species of closely related genera, indicating that the isolates are de facto members of the genus Listeria. Their overall fatty acid composition resembled that of other Listeria species, with quantitative differences in iso C15 : 0, anteiso C15 : 0, iso C16 : 0, C16 : 0, iso C17 : 0 and anteiso C17 : 0 fatty acid profiles. Phylogeny based on 406 core coding DNA sequences grouped these two isolates in a monophyletic clade within the genus Listeria. WGS-based average nucleotide identity and in silico DNA-DNA hybridization values were lower than the recommended cut-off values of 95 and 70 %, respectively, to the other Listeria species, indicating that they are founding members of a novel Listeria species. We suggest the name Listeriagoaensis sp. nov. be created and the type strain is ILCC801T (=KCTC 33909;=DSM 29886;=MCC 3285).

KEYWORDS:

Goa; L. goaensis; Listeria; haemolysis; new taxa

PMID:
30156532
DOI:
10.1099/ijsem.0.002980
[Indexed for MEDLINE]

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