A novel Gram-stain-negative, non-gliding, facultatively anaerobic and rod-shaped bacterium, designated X7T, was isolated from marine sediment taken from the coast of Weihai, China. Strain X7T grew optimally at 28-30 °C, at pH 7.0 and in the presence of 2-3 % (w/v) NaCl. Based on the 16S rRNA gene sequence analysis, strain X7T was a member of the genus Salinimicrobium and was most closely related to the species Salinimicrobium gaetbulicola with a 96.3 % 16S rRNA gene sequence similarity value. The major cellular fatty acids of strain X7T were anteiso-C15 : 0, iso-C15 : 0, anteiso-C17 : 1ω9c, iso-C17 : 1ω9c, C17 : 0 2-OH and iso-C17 : 0 3-OH. The major polar lipids of strain X7T were phosphatidylethanolamine, one unidentified phospholipid, two unidentified aminolipids and five unidentified lipids. The predominant respiratory quinone was MK-6, and the genomic DNA G+C content was 46.7 mol%. Phylogenetic, phenotypic, chemotaxonomic and genotypic results indicated that strain X7T represents a novel species of the genus Salinimicrobium, for which the name Salinimicrobium flavum sp. nov. is proposed. The type strain is X7T (=KCTC 42585T=MCCC 1H00115T).
Keywords: 16S rRNA gene; Salinimicrobium; Salinimicrobium flavum; marine sediment.