Format

Send to

Choose Destination

See 1 citation found using an alternative search:

Clin J Am Soc Nephrol. 2011 Aug;6(8):1903-11. doi: 10.2215/CJN.11571210. Epub 2011 Jul 22.

Oxidative stress and galactose-deficient IgA1 as markers of progression in IgA nephropathy.

Author information

1
Nephrology, Dialysis and Transplantation Unit, Regina Margherita Children's University Hospital, Piazza Polonia 94, 10127 Torino, Italy.

Abstract

BACKGROUND AND OBJECTIVES:

We assessed the activation of the oxidative stress pathway in patients with IgA nephropathy (IgAN), while evaluating the classic marker of the disease (galactose-deficient serum IgA1).

DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS:

Sera from 292 patients and 69 healthy controls from Italy and the United States were assayed for advanced oxidation protein products (AOPPs), free sulfhydryl groups on albumin (SH-Alb), and IgA1 with galactose-deficient hinge-region O-glycans (Gd-IgA1). Gd-IgA1 was detected by binding to Helix aspersa agglutinin (HAA) and expressed as total Gd-IgA1 or as degree of galactose deficiency relative to a standard Gd-IgA1 myeloma protein (%HAA).

RESULTS:

Sera from IgAN patients showed higher levels of Gd-IgA1, %HAA, and AOPPs, but lower levels of SH-Alb in comparison to that from healthy controls. Serum levels of AOPPs significantly correlated with serum Gd-IgA1 and %HAA. The relationship between these biomarkers and clinical features at sampling and during follow-up was assessed in 62 patients with long-term follow-up. AOPPs and %HAA correlated with proteinuria at sampling and independently associated with subsequent proteinuria. Levels of AOPPs correlated with rate of decline in renal function after sampling. The combination of a high level of AOPPs and a high level of %HAA associated with decline in estimated GFR.

CONCLUSIONS:

Serum levels of aberrantly glycosylated IgA1 are elevated and oxidative stress pathways are activated in patients with IgAN; the intensity of the stress correlated with expression and progression of the disease. We speculate that oxidative stress may modulate the nephrotoxicity of aberrantly glycosylated IgA1 in IgAN.

PMID:
21784819
PMCID:
PMC3156425
DOI:
10.2215/CJN.11571210
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center